Journal of Biomedical Optics, September/October 2009
J. Biomed. Opt. 14, 050505 (2009) (3 pages)
©2009 Society of Photo-Optical Instrumentation Engineers. All rights reserved.
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Simultaneous, noninvasive observation of elastic scattering, fluorescence and inelastic scattering as a monitor of blood flow and hematocrit in human fingertip capillary beds
Joseph Chaiken,1 †
Jerry Goodisman,1
Bin Deng,1
Rebecca J. Bussjager,2 and
George Shaheen2
1Syracuse University, CST1-014, Department of Chemistry and Department of Chemical and Biomedical Engineering, Syracuse, New York 13244-4100
2LightTouch Medical, Inc., 600 East Genesee Street, Suite 123, Syracuse, New York 13201
(Received: 20 May 2009; revised: 19 July 2009; accepted: 5 August 2009; published online: 7 October 2009)We report simultaneous observation of elastic scattering, fluorescence, and inelastic scattering from in vivo near-infrared probing of human skin. Careful control of the mechanical force needed to obtain reliable registration of in vivo tissue with an appropriate optical system allows reproducible observation of blood flow in capillary beds of human volar side fingertips. The time dependence of the elastically scattered light is highly correlated with that of the combined fluorescence and Raman scattered light. We interpret this in terms of turbidity (the impeding effect of red blood cells on optical propagation to and from the scattering centers) and the changes in the volume percentages of the tissues in the irradiated volume with normal homeostatic processes. By fitting to a model, these measurements may be used to determine volume fractions of plasma and RBCs. ©2009 Society of Photo-Optical Instrumentation Engineers
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