Journal of Biomedical Optics, March/April 2007
J. Biomed. Opt. 12, 020507 (2007) (3 pages)
©2007 Society of Photo-Optical Instrumentation Engineers. All rights reserved.
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JBO Letters
Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells
Steven Boutrus,1
Cherry Greiner,1
Derrick Hwu,1
Michael Chan,2
Charlotte Kuperwasser,3
Charles P. Lin,4 and
Irene Georgakoudi1,4 †
1Tufts University, Biomedical Engineering Department, Medford, Massachusetts 02155
2Tufts-New England Medical Center, Department of Radiation Oncology, Boston, Massachusetts 02111
3Tufts University School of Medicine, Department of Anatomy and Cellular Biology, Tufts-New England Medical Center, Molecular Oncology Research Institute, Boston, Massachusetts 02111
4Harvard Medical School, Massachusetts General Hospital, Wellman Center for Photomedicine, Boston, Massachusetts 02114
Received: 9 January 2007; revised: 14 February 2007; accepted: 16 February 2007; published: 24 April 2007The recent introduction of the in vivo flow cytometer for real-time, noninvasive detection and quantification of cells circulating in the vasculature of small animals has provided a powerful tool for tracking the roles of different types of cells in disease progression. We describe a portable version of the device, which provides the capability to: a) excite and detect fluorescence at two distinct colors simultaneously, and b) perform data analysis in real time. These advances improve significantly the utility of the instrument and provide a means of increasing detection specificity. As examples, we present the depletion kinetics of circulating green fluorescent protein (GFP)-labeled breast cancer cells in the vasculature of mice, and the specific detection of circulating hematopoietic stem cells labeled in vivo with two antibodies. ©2007 Society of Photo-Optical Instrumentation Engineers
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