Electronic excitation transfer as a probe of phase behavior in polymer composites
We use time-resolved fluorescence anisotropy decay measurements to watch the complete process of phase- separation of molecularly mixed polymer blends. Fluorescent chromophores are covalently incorpor...
Modeling single-molecule detection statistics
We present experimental results of single B-phycoerythrin molecule detection in a fluid flow at different sample introduction rates. A new mathematical approach is used for calculating the resulting b...
Fluorescence properties of indocyanin green: I. In-vitro study with micelles and liposomes
Proc. SPIE, Vol. 2980, 453 (1997);
doi:10.1117/12.273563
Online Publication Date: 8 April 2005
Conference Date: Sunday 09 February 1997
Conference Location: San Jose, CA, USA
Conference Title: Advances in Fluorescence Sensing Technology III
Conference Chairs: Richard B. Thompson
ICGis a tricarbocyanin fluorescent dye used in angiography. Several reportspoint out the advantage of ICG to fluoresce in thenear IR wavelength range enabling the imaging of deep tissues.If the fluorescence characteristics of ICG in buffer or proteinsolutions are described in vitro, little is known concerning thephysicochemical properties of ICG. This study aims to evaluate thephysicochemical and fluorescence spectral characteristics of ICG in aqueous solutionand in presence of micelles and liposomes. ICG exhibits atensioactive property and when incubated with micelles and liposomes tendsto be aggregated or embedded at the interface. The fluorescenceis very low in the aggregated form and is veryhigh when ICG is embedded at the interface and ashift of the emission peak toward longer wavelength is observed.Such in vitro study could contribute to a better understandingof some observed unusual properties of ICG in vivo.
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