Tissue birefringence of hypercholesterolemic rat liver measured with polarization-sensitive optical coherence tomography
We measure in vitro tissue birefringence in the liver of hypercholesterolemic rats with polarization-sensitive optical coherence tomography. Tissue birefringence is determined by measuring the phase r...
In vivo video-rate cellular-level full-field optical coherence tomography
Full-field optical coherence tomography (FF-OCT) capable of in vivo cellular-level imaging is demonstrated for nonscanning horizontal cross-sectional imaging. The system is based on a white light inte...
Weassess the accuracy of the Visante anterior segment optical coherencetomographer (AS-OCT) and present improved formulas for measurement of surfacecurvature and axial separation. Measurements are made in physical modeleyes. Accuracy is compared for measurements of corneal thickness (d1)and anterior chamber depth (d2) using-built-in AS-OCT software versus theimproved scheme. The improved scheme enables measurements of lens thickness(d3) and surface curvature, in the form of conic sectionsspecified by vertex radii and conic constants. These parameters areconverted to surface coordinates for error analysis. The built-in AS-OCTsoftware typically overestimates [mean±standard deviation(SD)]d1 by +62±4 µm and d2 by +4±88µm.The improved scheme reduces d1 (−0.4±4 µm) and d2 (0±49 µm) errorswhile also reducing d3 errors from +218±90 (uncorrected) to +14±123 µm(corrected). Surface x coordinate errors gradually increase toward the periphery.Considering the central 6-mm zone of each surface, the xcoordinate errors for anterior and posterior corneal surfaces reached +3±10and 0±23 µm, respectively, with the improved scheme. Those of theanterior and posterior lens surfaces reached +2±22 and +11±71 µm, respectively.Our improved scheme reduced AS-OCT errors and could, therefore, enhancepre- and postoperative assessments of keratorefractive or cataract surgery, includingmeasurement of accommodating intraocular lenses.