Age-related structural abnormalities in the human retina-choroid complex revealed by two-photon excited autofluorescence imaging
The intensive metabolism of photoreceptors is delicately maintained by the retinal pigment epithelium (RPE) and the choroid. Dysfunction of either the RPE or choroid may lead to severe damage to the r...
In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia
Multiphoton fluorescence lifetime imaging microscopy (FLIM) is a noninvasive, cellular resolution, 3-D functional imaging technique. We investigate the potential for in vivo precancer diagnosis with m...
Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis
J. Biomed. Opt., Vol. 12, 024013 (2007);
doi:10.1117/1.2717133
Published 24 April 2007
ABSTRACT
REFERENCES (34)
CITING ARTICLES
Hsin-Yuan Tan Chang Gung University, College of Medicine, Chang Gung Memorial Hospital, Department of Ophthalmology, Tao Yuan, Taiwan and National Taiwan University, College of Medicine and College of Engineering, Institute of Biomedical Engineering, Taipei, Taiwan
Sung-Jan Lin National Taiwan University, College of Medicine, National Taiwan University Hospital, Department of Dermatology, Taipei, Taiwan
Chen-Yuan Dong National Taiwan University, Department of Physics, Taipei, Taiwan
Thepurpose of this study is to demonstrate the application ofmultiphoton fluorescence and second harmonic generation (SHG) microscopy for theex-vivo visualization of human corneal morphological alterations due to infectiousprocesses. The structural alterations of both cellular and collagenous componentscan be respectively demonstrated using fluorescence and SHG imaging. Inaddition, pathogens with fluorescence may be identified within turbid specimens.Our results show that multiphoton microscopy is effective for identifyingstructural alterations due to corneal infections without the need ofhistological processing. With additional developments, multiphoton microscopy has the potentialto be developed into an imaging technique effective in theclinical diagnosis and monitoring of corneal infections.
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