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J. Biomed. Opt. / Volume 12 / Issue 2 / JBO Letters

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Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells

J. Biomed. Opt., Vol. 12, 020507 (2007); doi:10.1117/1.2722733

Published 24 April 2007

ABSTRACT

REFERENCES (9)

CITING ARTICLES

Steven Boutrus,¹ Cherry Greiner,¹ Derrick Hwu,¹ Michael Chan,² Charlotte Kuperwasser,³ Charles P. Lin,⁴ and Irene Georgakoudi^1,4
¹Tufts University, Biomedical Engineering Department, Medford, Massachusetts 02155
²Tufts-New England Medical Center, Department of Radiation Oncology, Boston, Massachusetts 02111
³Tufts University School of Medicine, Department of Anatomy and Cellular Biology, Tufts-New England Medical Center, Molecular Oncology Research Institute, Boston, Massachusetts 02111
⁴Harvard Medical School, Massachusetts General Hospital, Wellman Center for Photomedicine, Boston, Massachusetts 02114

Therecent introduction of the in vivo flow cytometer for real-time,noninvasive detection and quantification of cells circulating in the vasculatureof small animals has provided a powerful tool for trackingthe roles of different types of cells in disease progression.We describe a portable version of the device, which providesthe capability to: a) excite and detect fluorescence at twodistinct colors simultaneously, and b) perform data analysis in realtime. These advances improve significantly the utility of the instrumentand provide a means of increasing detection specificity. As examples,we present the depletion kinetics of circulating green fluorescent protein(GFP)-labeled breast cancer cells in the vasculature of mice, andthe specific detection of circulating hematopoietic stem cells labeled invivo with two antibodies.

©2007 Society of Photo-Optical Instrumentation Engineers

History:	Received 9 January 2007; revised 14 February 2007; accepted 16 February 2007; published 24 April 2007
DOI Link:	http://dx.doi.org/10.1117/1.2722733

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KEYWORDS and PACS

Keywords

biomedical optical imaging, fluorescence, cellular biophysics, biological organs, cancer, tumours, mammography

PACS

87.63.Lk
Visible radiation: diaphanography, transillumination, laser imaging in medicine
87.17.-d
Cellular structure and processes
87.19.Xx
Diseases
YEAR: 2007

JOURNAL DATA

ISSN:

1083-3668 (print) 1560-2281 (online)

Publisher:

SPIE

REFERENCES (9)

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H. M. Shapiro, Practical Flow Cytometry, 3rd ed., Wiley-Liss, New York (1995).
J. P. Pawley, Handbook of Biological and Confocal Microscopy, 2nd ed., Plenum, New York (1995).
J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, “In vivo flow cytometer for real-time detection and quantification of circulating cells,” Opt. Lett. 29, 77–79 (2004). [ISI] [MEDLINE]
I. Georgakoudi, N. Solban, J. Novak, W. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: A new method for enumerating circulating cancer cells,” Cancer Res. 64, 5044–5047 (2004). [ISI] [MEDLINE]
D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature (London) 435, 969–973 (2005). [MEDLINE]
X. Wei, D. A. Sipkins, C. M. Pitsillides, J. Novak, I. Georgakoudi, and C. P. Lin, “Real-time detection of circulating apoptotic cells by in vivo flow cytometry,” Mol. Imaging 4, 415–416 (2005). [MEDLINE]
J. Novak, “Development of the in vivo flow cytometer,” PhD Thesis, Massachusetts Institute of Technology, Boston, MA (2004).
A. F. Chambers, A. C. Groom, and I. C. MacDonald, “Dissemination and growth of cancer cells in metastatic sites,” Nat. Rev. Cancer 2, 563–572 (2002). [MEDLINE]
K. Ikuta and I. L. Weissman, “Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation,” Proc. Natl. Acad. Sci. U.S.A. 89, 1502–1506 (1992). [MEDLINE]

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