Computer-based tracking of single sperm
This paper describes a robust single sperm tracking algorithm (SSTA) that can be used in laser optical trapping and sperm motility studies. The algorithm creates a region of interest (ROI) centered ab...
simEye: computer-based simulation of visual perception under various eye defects using Zernike polynomials
We describe a computer eye model that allows for aspheric surfaces and a three-dimensional computer-based ray-tracing technique to simulate optical properties of the human eye and visual perception un...
Recentlythe psychophysical compensation comparison method was developed for routine measurementof retinal stray light. The subject's responses to a seriesof two-alternative-forced-choice trials are analyzed using a maximum-likelihood (ML) approachassuming some fixed shape for the psychometric function (PF). Thisstudy evaluates the reliability of the method using Monte-Carlo simulations.Various sampling strategies were investigated, including the two-phase sampling strategythat is used in a commercially available instrument. Results aregiven for the effective dynamic range and measurement accuracy. Theeffect of a mismatch of the shape of the PFof an observer and the fixed shape used in theML analysis was analyzed. Main outcomes are that the two-phasesampling scheme gives good precision (Standard deviation=0.07 logarithmic units onaverage) for estimation of the stray light value. Bias isvirtually zero. Furthermore, a reliability index was derived from theresponses and found to be effective.
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